論文

Nowadays, innovative therapies for tissue engineering and regenerative medicine have been created and a large number of cells with high viability are cultured for these therapies. In the cell detachment method with common protein-degrading enzymes, the cell membranes are damaged at the detachment. On the other hand, the cell detachment method with ultrasonic vibration can detach without damage of cell membranes. For instance, the adherent cells on the culture dish are detached from the bottom of the dish by using sweep vibration without enzymes. The deformation of the culture dish, the acoustic streaming-driven shear flow, the acoustic pressure on a boundary of different materials having different acoustic impedance, and the sloshing of the liquid are candidates for detachment factor. However, details of the dominant conditions for cell detachment are not obvious. Therefore, in this study, we evaluated the effects of each factor on cell detachment from experiments and theory. As results, the deformation of the culture dish or the acoustic streaming-driven shear flow does not have sufficient force to detach the cells, while the pressure between the different acoustic impedance and the sloshing of the liquid are sufficient to detach the cells from reference. That is, these results indicate that the different acoustic impedance and the sloshing of the liquid are dominant factors for cell detachment with ultrasonic vibration.