総説・解説等

In the originally published version of this article, an incorrect version of Figure 4 was included due to an error during the final submission process. Figure 4 has been replaced with the correct version, as shown below. The original article has been corrected accordingly. This correction does not affect the results, discussion, or conclusions of the article. The authors apologize for any inconvenience caused. The original article has been corrected. Correct Fig. 4: (Figure presented.) Concentrations and relative compositions of elements measured using EDXRF in the ambient PM collected using the PVA and PTFE filters. Elements are listed in descending order of concentration

Particulate matter (PM) is among the major air pollutants suspended in the atmosphere. PM2.5 has a particle size of 2.5 µm; it is known to cause inflammation, especially in the respiratory tract and skin. Since the skin acts a primary barrier against harmful environmental substances that may enter the body, it is highly exposed to PM2.5 present in the environment. However, the adverse health effects of PM2.5 exposure on human skin have not been accurately examined due to the lack of a system that expos-es human epidermal tissue to the actual environmental concentration of PM2.5. In this study, we developed an air–liquid interface exposure system for exposing cultured human 3D epidermis and cornea to PM2.5 collected through cyclonic separation. PM2.5 suspension was nebulized in an acrylic chamber, and the resulting mist was pumped through a diffusion dryer into a glass exposure chamber. A particle counter was connected to the exposure chamber to continuously measure the spatial mass concentration of PM. Human 3D epidermis was cultured in the exposure chamber. Exposure of the human 3D epidermis to PM aerosol increased interleukin-8 release into the media around 50 µg/m3. Mass concentrations above 100 µg/m3 caused cell death. Furthermore, a human corneal model showed similar responses against PM2.5 exposure as 3D epidermis. The air–liquid interface exposure system developed in this study is considered useful for evaluating the health effects induced by environmental PM2.5 and can be used as an alternative to experiments involving actual human or animals.