Imaging live cells with fluorescent proteins has long been a crucial technique for understanding cellular behavior. While bioluminescent proteins offer several advantages over fluorescent proteins, the limited availability of color variants has made it difficult to observe multiple targets simultaneously. Now, researchers from SANKEN (The Institute of Scientific and Industrial Research, Osaka University) and Keio University have developed a groundbreaking method to expand the color palette of bioluminescent protein to 20 distinct colors, enabling advanced simultaneous multi-color imaging.
