Challenge Grant: Development of Cell-Selective Cryotherapy Based on Disease-Specific Temperature Response Mechanisms

In the 2025 research plan, we aimed to establish the theoretical foundation for cell-selective cryotherapy by collecting and analyzing fundamental data on temperature sensitivity among different cell types. As originally planned, we focused on congenital giant melanocytic nevus (GCMN), for which no established treatment currently exists, and investigated the conditions required to enable cell-selective cryotherapy as well as the mechanisms leading to selective cell death.

In collaboration with the University of Tokyo and Kochi University, we have successfully established an experimental system that enables real-time observation of mitochondrial changes during the cooling process, and preparations are in place to analyze the relationship between temperature control and mitochondrial responses. By continuing these analyses, we aim to clarify the factors underlying cell selectivity induced by temperature control and potentially identify applications of temperature-controlled therapies for other pathological conditions.

In fiscal year 2026, we will further elucidate the mechanisms of cell selectivity induced by temperature control. In addition, we will expand the target diseases, including keloids, and observe cellular responses to temperature control in these conditions to explore new therapeutic possibilities.