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Capillary electrophoresis-mass spectrometry (CE-MS) has gained a great deal of attention as a powerful tool for the analysis of charged compounds. This approach offers high-resolution, high-sensitivity, and wide versatility. Over the past three decades, several CE-MS methods have been developed and have made significant advances in analytical chemistry. In this chapter, the general strategies and principles of CE-MS methods will be discussed, including CE separation modes, interface techniques, mass spectrometry, new techniques to increase the throughput capability of CE-MS analysis, metabolomics, and even single-cell analysis, which has received much attention in recent years. Finally, general conclusions and perspectives are provided.

Capillary electrophoresis-mass spectrometry (CE-MS) has proven to be useful for metabolomics studies. Charged metabolites are first separated by CE based on charge and size and are subsequently selectively detected using MS. The major advantages of CE-MS are its high resolution and the fact that almost any charged species can be analyzed by two methods, both cationic and anionic. This technique can readily be applied to various types of biological samples originating from bacteria, plants, mammals, and body fluids. This chapter highlights detailed practical procedures for using this technology.

Capillary electrophoresis-mass spectrometry (CE-MS) has been developed as a powerful tool in the analysis of charged compounds. To simultaneously analyze free amino acids, an electrolyte with low pH was used to positively charge all of the amino acids. In this condition, all protonated amino acids migrated toward the cathode in CE and then were sensitively and selectively detected by MS. This method is simple, rapid, and selective and could readily be applied to the analysis of free amino acids in various samples. In this chapter, the detailed procedure to analyze amino acids using CE-tandem mass spectrometry (MS/MS) is described.

生体内には糖、有機酸、アミノ酸など数千種にも及ぶ低分子が存在し、ほとんどは酵素などの代謝活動によってつくり出された代謝物質である。細胞の働きを理解しようとするとき、ゲノム解析やプロテオーム解析に加え、代謝物質の網羅的解析（メタボローム解析）はきわめて重要となる。本書はこのメタボローム研究をいち早く紹介する待望のレビュー集。