Published papers

様々な組成比を有するアクリルアミドとメタクリル酸の共重合体からなる微粒子を作製した。これらの微粒子が血小板に与える作用を細胞内カルシウム濃度と膜の流動性の観点から検討を行った。

A cell adhesive peptide, Arg-Gly-Asp-Ser (RGDS), enhances leucocyte response to stimuli when insolubilized or conjugated with proteins. In order to investigate the contribution of the microsphere structure to leucocyte response, RGDS was immobilized onto several polymeric microspheres. The respiratory burst of polymorphonuclear leucocytes (PMNs) contacting microspheres was enhanced by RGDS in cases where it was immobilized on polystyrene and, more effectively, a positively charged one. This was not observed in polyacrylamide-based or negative-charged microsphere systems. These results demonstrate that the property of supports strongly affects the regulation of the PMN response via an adhesive interaction. © 1994.

The adhesive interaction of cells with extracellular matrix components is essential for a variety of cellular functions, and is frequently mediated by a tetra peptide, Arg‐Gly‐Asp‐Ser (RGDS), located within fibronectin and other proteins. In this study, the RGDS‐mediated activation of polymorphonuclear leukocytes accompanied by phagocytosis was investigated using monodisperse polymeric microspheres carrying RGDS. The parent and Arg‐Gly‐Glu‐Ser (RGES)‐carrying microspheres, which have no adhesion activity, were employed as controls. The ingestion of microspheres into PMN was not enhanced by immobilizing RGDS. However, PMNs exhibited unique oxygen consumption and enhanced liberation of reactive oxygen when RGDS‐carrying microspheres were phagocytosed. These PMN responses disappeared with the addition of soluble RGDS. Furthermore, cytochalasin D, which inhibits actin polymerization, showed a marked inhibitory effect on oxygen consumption in the RGDS‐carrying microsphere system, as compared with those in other systems. These findings show that RGDS‐carrying microspheres induced the biospecific activation of PMNs by the signal transduction via RGDS‐integrin binding without alteration in the degree of phagocytosis. © 1994 John Wiley & Sons, Inc. Copyright © 1994 John Wiley & Sons, Inc.

コア部に電荷を持ち、感温性のヘアを表面に有するコアシェル微粒子の泳動挙動を測定し、電荷導入によるヘア層の状態変化について検討を行った。

ポリビニルアルコールの表面状態を変化させるために、種々な熱処理を行った。次にこの表面と接触した血液成分の状態変化を測定した。その結果、適当な可塑剤量、熱処理温度、および時間の時に優れた生体適合性を示すような表面状態になることがわかった。

細胞接着性のテトラペプチド（ＲＧＤＳ）を結合させた微粒子は白血球に対して、特異的に接着し、活性化させることがわかった。

転写活性を制御する因子が特異的に結合する二本鎖DNA部分を微粒子に結合させてアフィニティ微粒子を作製した。この微粒子を用いて細胞から転写活性因子の単離を行ったところ、高収率で回収できた。

タンパク質は両性高分子電解質であるために様々な等電点を持つ。カチオン性の微粒子は静電的な引力により、アニオン性のタンパク質を高収率で回収することができた。

様々な化学組成および形態を有する微粒子を作製し、顆粒球による貪食作用を調べた。ある親疎水性の混合割合の時に貪食作用が抑制される結果を得た。補体成分の活性化の抑制によるものと考えられた。

コアとシェルを持つ微粒子のシェル部の変化によりコア部と外部との透過性を制御することが出来る。ここでは感温性シェルを持つ微粒子について電子の伝達を制御することに成功した。

博士（工学） 甲第5400号

様々な高分子物質からなる小口径のチューブを用いて、動脈-静脈シャントを行い、主に血小板粘着挙動を調べた。

Acrylamide, glycidyl methacrylate, and methylene bisacrylamide were copolymerized in an aqueous ethanol to form monodisperse hydrogel latex particles. Antibody of C-reactive protein (CRP) was immobilized on the particles through a hydrophilic spacer. The resulting particles were moderately stable, and the agglutination was proportional to the concentration of CRP. They were little susceptible to the nonspecific adsorption of proteins and the aggregation by rheumatoid factor. Therefore, it was concluded that the hydrogel particles are a promising carrier of antibodies for latex diagnosis. © 1993, The Society of Polymer Science, Japan. All rights reserved.

感温性のヘアを表面に有する微粒子の泳動挙動を測定し、ヘア層の状態を柔らかさの観点からとらえる試みを行った。

ポリスチレンの表面およびアクリルアミドとアクリル酸の共重合体表面が血小板に与える影響をその代謝機能変化から検出し、ポリマー鎖の動的な状態が重要な因子であることがわかった。

感温性ゲル微粒子への様々なタンパク質の吸着現象について検討を行った。

粒子径の揃ったヒドロゲル微粒子を沈殿重合により作製し、その生成過程の検討と更なる改良を行った。

ポリウレタンを低温プラズマ処理し、表面へのアクリルアミドのグラフト重合を行った。この改質により表面へのタンパク質の吸着および血小板の粘着は著しく抑制された。

Microspheres having cell adhesion activity were prepared by immobilizing a tetrapeptide Arg-Gly-Asp-Ser (RGDS) onto the surface. Phagocytosis of the microspheres by leukocytes was not enhanced by immobilizing RGDS, but specific oxygen consumption was observed during the incubation of the RGDS-carrying microspheres with leukocytes. This was enhanced by a spacer introduced between RGDS and the microsphere. These results suggested that the immobilized RGDS could transduce the adhesion signal, resulting in the activation of leukocytes without enhancing phagocytosis of the microspheres. Furthermore, it was found that soluble RGDS did not induce oxygen consumption at all by itself. This result suggests that RGDS must be immobilized on solid carriers for the specific activation of leukocytes. Preparation and Evaluation of Polymer Microspheres with Cell Adhesion Activity. © 1993, The Society of Polymer Science, Japan. All rights reserved.