2019/11/29
RIKEN
Keio University School of Medicine
A joint research group, including Senior Research Scientist Junshi Yazaki and Team Leader Osamu Ohara of the Laboratory for Integrative Genomics at the RIKEN Center for Integrative Medical Sciences, Team Leader Katsuyuki Shiroguchi of the Laboratory for Cellular Systems Modeling at the RIKEN Center for Biosystems Dynamics Research, and Professor Masayuki Amagai of the Keio University School of Medicine, has developed a new protein labeling method called the "protein-DNA conjugate construction method (HaloTag protein barcoding method)" and has successfully quantified proteins using a next-generation sequencer.
The results of this research are expected to impact basic medical sciences, particularly medical proteomics research, and contribute to high-precision preventive medicine.
In this study, the joint research group developed a new protein labeling method that utilizes a click chemistry reaction and the binding tag protein HaloTag to create a protein-DNA conjugate by covalently bonding a DNA molecule to a target protein in a one-to-one ratio, and then quantifies the protein molecules that bind to this target protein using the DNA. This method has 100 to 1,000 times higher sensitivity than the conventional luciferase method and has also successfully reproduced previously reported protein-protein interactions. Furthermore, in the detection of anti-desmoglein 3 antibodies using desmoglein 3, the antigen for the autoimmune disease pemphigus, it demonstrated more than 100 times the sensitivity of the conventional ELISA method.
This research was published in the online edition of the British scientific journal "Nucleic Acids Research" on November 22 (November 22, JST).
Please see below for the full press release.