Keio University

Shared Equipment Room

Microscopy Room

Confocal Laser Scanning Microscope

Model: Olympus FV3000

A confocal microscope system capable of high-sensitivity, high-speed, and super-resolution imaging. It features multi-color simultaneous imaging via spectral technology, tiling for merging multiple images, time-lapse for live imaging, autofocus functions, and 3D image construction.

Cell Culture Room

Clean Bench

Model: Sanyo MCV-B131F

A clean bench equipped with a low-pressure loss HEPA filter, all-stainless steel (SUS-304) interior, distributed suction system, and an air curtain mechanism.

Liquid Nitrogen Dewar Flask

Model: Locator 8 Plus

An insulated aluminum cryopreservation container equipped with a monitoring device that uses ultrasonic waves to detect the liquid nitrogen level.

Molecular Biology Equipment Room

Automated Cell Analyzer (FACS)

Model: BD FACS ARIA III

A device that measures scattered light and fluorescence obtained by irradiating cells or particles flowing in a liquid with laser light, analyzes the characteristics of individual cells or particles, and sorts specific cells. Equipped with 5 lasers, it enables high-performance analysis and high-performance sorting.

Automated Cell Analyzer (FACS)

Model: BD FACS Symphony A5

Automated Cell Analyzer (FACS)

Model: BD FACS Celesta

Real-Time PCR

Model: BIORAD CFX96

Features outstanding temperature uniformity, reaching within ±0.4°C of the target temperature within 10 seconds, enabling uniform amplification regardless of well position.

In the optical section, an optical shuttle equipped with a long-life 6-LED and 6-photodiode detector scans all wells, allowing for efficient, high-sensitivity fluorescence detection with the shortest optical path and no crosstalk between wells.

Utilizes the most versatile 96-well format and allows for detection of up to 5 fluorescence colors.

Single-Cell Gene Expression Analyzer

Model: 10x GENOMICS Chromium Controller

A droplet generation device for performing single-cell RNA sequencing (scRNA-seq) and scATAC-seq. It enables gene expression analysis at the single-cell level, epigenomic analysis, and repertoire analysis of lymphocyte antigen receptors.

Darkroom / Cold Room / Shaking Incubator Room

Darkroom

Panoramic view

Cold Room

Panoramic view

Shaking Incubator (with Universal Rack)

Model: NBS Innova 4230

A benchtop shaker that can be stacked up to two units high, suitable for high-speed shaking culture of animal cells, yeast, and bacteria such as E. coli. Computer control allows settings from 20°C below room temperature (minimum 4°C) to 80°C in 0.1°C increments, making it useful for culturing various cell types.

Recombinant DNA Laboratory

Safety Cabinet

Model: Panasonic MHE-S1301A2-PJ

A Class II Type A2 biological safety cabinet.

Equipped with a low-pressure loss HEPA filter and stainless steel interior. Dust collection efficiency of 99.99%.

Cleanliness Class 100. An inverter-controlled fan motor and wind speed sensor allow for automatic fan speed control, maintaining a consistently stable and appropriate airflow balance.

CO2 Incubator

Model: SANYO MCO-19AIC (UV)

A CO2 cell culture device with adjustable settings for temperature (ambient +5°C to 50°C) and CO2 concentration (0 to 20%).

Anaerobic Chamber

Model: Sheldon Bactron 300

A device for culturing anaerobic microorganisms. Since the incubator is temperature-controlled and separated by a door, work inside the chamber can be performed at room temperature.

Centrifuge Room

Ultracentrifuge

Model: Hitachi CP80WX

Maximum rotation speed of 80,000 rpm and maximum centrifugal acceleration of 615,000 x g.

Temperature setting range from 0°C to 40°C with thermo-module cooling.

NMR Room

FT-NMR (Avance Neo 600)

Model: Bruker Avance Neo 600

By observing the magnetic resonance of 1H, 13C, and 15N nuclei in molecules within a solution, information regarding the state of electrons surrounding the nuclei and chemical bonds can be obtained.

For example, even for the same type of element, different NMR signals are observed from atoms at different positions within a molecule. The greatest feature is the ability to observe and analyze each atom within a molecule distinctly.

By analyzing NMR spectra, structural analysis at the atomic level is possible, including structure determination of synthetic and natural products, three-dimensional structural analysis of proteins, and analysis of intermolecular interactions and mobility.

FT-NMR (ECP600)

Model: JEOL JNM-ECP600

Primarily used to obtain information regarding hydrogen and carbon in compounds. It is the most important instrument for confirming and determining the structure of organic compounds.

GC-MS Room

Magnetic Sector GC-MS (MS700)

Model: JEOL JMS-700

A device that obtains information on molecular mass and molecular formulas through low and high-resolution mass measurement.

Precision Instrument Room

FT-IR

Model: JASCO FT/IR-4700

A Fourier Transform Infrared Spectrophotometer. It uses corner cube mirrors for the moving mirror inside the interferometer, and the interferometer is digitally controlled.

Capable of wide-range measurements from near-infrared to far-infrared.

Quadrupole GC-MS

Model: JEOL JMS-Q1500GC

Allows for easy low-resolution mass measurement (introduction via GC only). A device for obtaining molecular mass information.

Time-of-Flight MS (TOF-MS)

Model: Shimadzu MALDI-8020

A versatile high-resolution, high-precision mass spectrometer that combines Matrix-Assisted Laser Desorption/Ionization (MALDI), capable of ionizing a wide range of samples, with a Time-of-Flight (TOF) mass analyzer that has a wide measurable molecular weight range.

LC-MS Room

LC-MS/MS

Model: Shimadzu 8050

A triple quadrupole mass spectrometer.

Capable of high-speed, high-sensitivity quantification, simultaneous analysis of multiple components in a short time, and simultaneous qualitative and quantitative analysis.